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Additionally, LY46 showed higher expression of genes encoding PHGDH, PSAT, and PSP enzymes that link glycolysis to serine biosynthesis, potentially impacting downstream sulfur amino acid (like cysteine) and glutathione production

After fixation, the coverslips were washed 3 times with 0.1 M PBS for 10 min each, then blocked with 0.1 M phosphate-buffered saline (PBS) containing 0.3% Triton X-100 (Sigma, USA) and 10% Donkey Serum (Genetex) for 1.5 h at room temperature

Creatine, melatonin, phosphatidylcholine and carnitine can help to conserve methylation by providing extra methyl donors

Dysregulation of the circadian rhythm also occurred at the protein level